| Abstract Scope |
Bicontinuous interfacially jammed emulsion gels (“bijels”) consist of two interpenetrating continuous phases, with each being filled with a liquid. Biocompatible bicontinuous bijels-derived structures may be made, which can have interconnected channels that are desirable in tissue engineering as the channels can provide space for cell proliferation and migration. A solvent transfer-induced phase separation-based technique was developed by previously, which could make biocompatible bijels-derived hybrid structures. In this study, bijels-derived porous hexanediol diacrylate membranes of different channel sizes (20-100um) were fabricated for studying cell behaviors on/in scaffolds. Human dermal fibroblasts and osteoblastic MC 3T3 cells were cultured separately on bijels-derived porous hexanediol diacrylate for up to 28 days. Both cells showed high cell viability and good cell proliferation on scaffolds. Cells could migrate and proliferate well in channels of membranes having average channel sizes larger than 50um. In membranes with smaller channel sizes, cells only exhibited partial migration into channels. |