| About this Abstract |
| Meeting |
2020 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
Application of Titanium Dioxide Nanotubes and Lattice Light-sheet Microscopy in Establishing Early-stage Cellular Response Mechanisms |
| Author(s) |
Jevin G. Meyerink, Scott Wood, Jeremy Mercuri, Robert Anderson, Brandon Scott, Grant Crawford |
| On-Site Speaker (Planned) |
Jevin G. Meyerink |
| Abstract Scope |
There is a need for novel titanium surface treatments, like TiO2 nanotubes, to promote osteogenesis on orthopedic implants, yet, the exact mechanisms by which this nanostructure influences adhesion dynamics and how those dynamics subsequently mediate intracellular signaling pathways to dictate cell function, remains unclear. The purpose of this study is to use advanced live-cell imaging techniques to understand how nanostructured surfaces influence early-stage cell adhesion. We investigate the influence of TiO2 nanotube structure on initial cell-TiO2 nanotube interaction of various cell types (pre-osteoblasts, macrophages, mesenchymal stem cells) via lattice light sheet microscopy (LLSM). Taking advantage of recent advances in LLSM, we are capable of rapid volumetric image acquisition during early-stage adhesion and long imaging duration (hours to days). In addition, we evaluate the requirement for specific proteins in the nanotube diameter-modulation of osteogenic and phagocytic signaling dynamics, with the goal of correlating these important dynamic processes to TiO2 nanotube diameter. |
| Proceedings Inclusion? |
Planned: Publication Outside of TMS |