| About this Abstract |
| Meeting |
MS&T24: Materials Science & Technology
|
| Symposium
|
3D Printing of Biomaterials and Devices
|
| Presentation Title |
A Novel Horizontal Flow Bioreactor for Studying Cancer Progression |
| Author(s) |
Dinesh Katti, Kalpana S. Katti, Sharad Jaswandkar, Preetham Ravi |
| On-Site Speaker (Planned) |
Dinesh Katti |
| Abstract Scope |
Prostate cancer bone metastasis is a leading cause of cancer-related deaths in men. The treatment of advanced-stage prostate cancer remains largely ineffective. Interstitial fluid flow has been shown to influence cell proliferation. The understanding of the impact of interstitial fluid flow on cancer cell growth and migration is limited. In this study a bioreactor is designed to examine the effect of fluid flow on late-stage prostate cancer migration. High flow rates decreased DNA content in PC3-type prostate cancer cells and induced apoptosis via TGF-β1 activation. The migration rate of these cells was evaluated under fluid flow conditions, revealing that bone upregulation of CXCR4 levels led to increased MMP-9 levels. Both αVβ3 integrins and MMP-9 levels were upregulated under fluid-flow conditions, contributing to increased migration. The study suggests that interstitial flow-induced shear stress may be crucial in regulating cancer cell migration, offering potential for controlling tumor progression and developing new therapies. |