About this Abstract |
Meeting |
MS&T24: Materials Science & Technology
|
Symposium
|
Controlled Synthesis, Processing, and Applications of Structural and Functional Nanomaterials
|
Presentation Title |
Polymeric Supraparticles with Plasmonic Enhancement for Advance Diagnostic Performance |
Author(s) |
Gabriel Aguirre Cruz, Lia A. Stanciu |
On-Site Speaker (Planned) |
Lia A. Stanciu |
Abstract Scope |
Plasmonic-enhanced fluorescence offers outstanding sensitivity in diagnostics, especially when using anisotropic plasmonic particles with powerful resonances. We designed a polymeric 500 ± 10nm supraparticle resonating with spherical and anisotropic Au-based plasmonics (60-20nm), coated by a nanometer-scale SiO2 spacer (3-20 nm). Using TEM and High-Resolution Fluorescence Microscopy, we quantified the effect of spacer thickness and particle geometry on fluorescence output. STEM/EELS identified optimal configurations for plasmonic hotspot formation.
Fluorescent core-shell particles were coupled with aptamer sequences for high-sensitivity detection of oral pathogens S. Sanguis, S. Mutans, and P. Gingivalis, demonstrating excellent detection limits, selectivity, specificity, and stability. Our work overcomes limitations of two-dimensional systems by creating a three-dimensional fluorescent plasmonic bead-based structure compatible with traditional and advanced immunoassay platforms. |