| Abstract Scope |
Herein; we report a versatile approach for engineering injectable and biomimetic nanofiber microspheres (NMs) with tunable sizes, predesigned structures, and desired compositions via gas bubble-mediated co-axial electrospraying. The sizes and structures of NMs were controlled by adjusting processing parameters, including air flow rate, applied voltage, distance, and spinneret configuration in the co-axial setup. Importantly, unlike the self-assembly method, this technique can fabricate NMs from any material feasible for electrospinning or other nanofiber fabrication techniques.Varying parameters, such as airflow rates and applied voltages, enables a tunability that is yet to be reported in engineering NMs. We also demonstrated that NMs serving as cell carriers could enhance expansion and differentiation, suggesting the potential use for stem cell therapies. The in vitro and in vivo studies showed that the open porous architecture of the NMs provided an ideal matrix for cellular infiltration and integration with host tissue. |