About this Abstract |
Meeting |
MS&T23: Materials Science & Technology
|
Symposium
|
Next Generation Biomaterials
|
Presentation Title |
Exploring the Potential of Carvacrol for Orthopaedic Applications: In Vitro Gene Expression and In Vivo Studies |
Author(s) |
Aditi Dahiya, Susmita Bose |
On-Site Speaker (Planned) |
Aditi Dahiya |
Abstract Scope |
Phytochemicals such as Carvacrol is an active agents found in oregano and thyme, known for their antibacterial, anti-inflammatory, and anticancer activity. The effects of carvacrol on bone health are not well explored. This study investigates the biological properties of carvacrol on calcium phosphate matrices. The results revealed that the carvacrol-loaded substrate effectively inhibits osteoclast cell viability by ~50% after 21 days of culture. The co-culture studies of human mesenchymal stem cells (hMSC) and monocytes showed enhanced fibroblastic extensions of osteoblast cells and reduced osteoclast cell population on carvacrol-loaded hydroxy apatite substrate. Also, in comparison to the control, a threefold higher osteoblast-related gene expression of BGLAP was observed for the functionalized scaffold, indicating carvacrol-enhanced differentiation of hMSC into osteoblast. RANKL expression is significantly reduced, indicating that carvacrol down-regulates osteoclastogenesis. The results suggest carvacrol's efficiency for future therapeutic strategies for bone-related disorders. |