| About this Abstract |
| Meeting |
MS&T23: Materials Science & Technology
|
| Symposium
|
Next Generation Biomaterials
|
| Presentation Title |
Protein Microarrays on a Nonfouling Polymer Brush for Point of Care Detection |
| Author(s) |
Ashutosh Chilkoti |
| On-Site Speaker (Planned) |
Ashutosh Chilkoti |
| Abstract Scope |
I will describe a point-of-care diagnostic in which all reagents are inkjet-printed printed and stored on a “non-fouling”—protein and cell resistant—polymer brush. The D4 assay involves four sequential events: (1) Dispense (droplet of blood); (2) Dissolve (printed reagents on chip); (3) Diffuse (across surface); and (4) Detect (binding event). The D4 assay yields quantitative results with picomolar sensitivity within an hour. Upon application of a drop of blood to the D4 chip, analyte capture and detection on the D4 chip occurs automatically, generating an image of fluorescent spots that is imaged by a hand-held detector and quantified by an on-board App. I will discuss examples of rapid diagnostic tests we have developed on the D4 platform including a test for Ebola from blood that beats the performance of a PCR based test, another to diagnose SARS-CoV-2 from viral transport media that also enables discrimination between different variants. |