| About this Abstract |
| Meeting |
2022 TMS Annual Meeting & Exhibition
|
| Symposium
|
2022 Technical Division Student Poster Contest
|
| Presentation Title |
SPG-16: Study of Anti-inflammatory miRNA146a Loading on Cerium Oxide Nanoparticles with Different Surface Facet |
| Author(s) |
Yifei Fu, Elayaraja Kolanthai, Craig Neal, Udit Kumar, Carlos Zgheib, Kenneth Liechty, Sudipta Seal |
| On-Site Speaker (Planned) |
Yifei Fu |
| Abstract Scope |
Nowadays, noncoding miRNA-based therapy has shown remarkable performance in the bio-medical field. However, miRNA delivery to the target site presents a great challenge in medicine due to its rapid degradation, low cell uptake, presence of endonucleases in blood, and negative polarity. To overcome these obstacles, many strategies have been applied, such as blocked nucleic acid integrating, polymer/lipid encapsulation, and nanoparticles conjugation to improve miRNA stability/delivery. In this study, the amine-modified miRNA-146a was conjugated to cerium dioxide nanoparticles with different surface facets by the bioconjugation method using carbonyldiimidazole as a linker. The hydrodynamic diameter and zeta-potential of nanoparticles are characterized by dynamic light scattering. Conjugated miRNAs on cerium dioxide nanoparticles were quantified by a commercial miRNA quantification assay and gel electrophoresis. Furthermore, miRNA-146a loading capacity and enzymatic activity were analyzed and results were compared between nanoparticles with different surface facets. The presentation will conclude with in vitro wound healing studies. |
| Proceedings Inclusion? |
Undecided |
| Keywords |
Nanotechnology, Biomaterials, |