| About this Abstract |
| Meeting |
2025 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
The Influence of Buffer Composition and Surface Topography on the Antimicrobial Properties of Copper |
| Author(s) |
Rebecca Reiss, William T. Kluck, Kaitlyn M. Betz, Daniela P. Hirsch, Terry C. Lowe |
| On-Site Speaker (Planned) |
Rebecca Reiss |
| Abstract Scope |
The antimicrobial nature of copper and copper alloys is well established, but our understanding of the precise mechanisms of toxicity continues to evolve. Few studies have focused on how the interaction of proteins, bacteria, and buffer constituents at the copper/buffer interface can influence the antimicrobial response, particularly for short-duration contact killing. The Advanced Small Drop Assay (ASDA) was used to compare antibacterial testing with Phosphate Buffered Saline (PBS), commonly used as a diluent in antimicrobial testing, versus chloride-free Butterfield’s Phosphate Buffer (BPB). Bacteria were more rapidly killed in PBS than in BPB when exposed to the copper surfaces. Although both buffers produced nanoflowers, these nanostructures are different when exposed to PBS or BPB and have only limited effects on their antimicrobial properties. Etched copper surfaces increased antibacterial in the PBS and BPB buffers. These results indicate the importance of the effects of buffer composition on short-duration antimicrobial testing, the utility of the ASDA to increase the throughput of antibacterial testing, and provide clues that will help resolve the mechanism(s) of toxicity. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Characterization, Nanotechnology |