| About this Abstract |
| Meeting |
2022 TMS Annual Meeting & Exhibition
|
| Symposium
|
Biological Materials Science
|
| Presentation Title |
Mimicking the Glioblastoma Cells' Microenvironment via Anisotropic Sub-microscaled Surface Patterns and Enabling Directional Adhesion of Filopodia on Metallic Biomaterials |
| Author(s) |
Benay Uzer-Yilmaz |
| On-Site Speaker (Planned) |
Benay Uzer-Yilmaz |
| Abstract Scope |
Glioblastoma (GBM) is the most aggressive brain tumor type and contact guidance via aligned-nanoscaled white matter tracts plays crucial role for their invasion. In this study, this microenvironment was mimicked by tensile loading 304L stainless steels up to 5% and 25% strains. Microstructural evolution and topography of surface relief were analyzed via AFM and SEM. Cell morphology was investigated with SEM and immunofluorescence technique. Spacing of directional surface patterns decreased down to submicro-scale with deformation. Kinking of filopodia was captured on 25% deformed-sample evidencing constraint induced by deformation markings and exerted contact forces. Findings suggest that deformation markings can block filopodia movement and suppress cells’ tendency for invasion. Filopodia elongation orthogonal to aligned submicro-scaled markings further suggests that anisotropic topographical cues could be used for directing cells along desired pathways. These results can be used to design metallic implants for therapeutic purposes targeting migration pathways of GBMs and controlling invasion. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Environmental Effects, Characterization |