| About this Abstract |
| Meeting |
2020 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
Inkjet Printed Nano-patterned Aptamer-based Sensors for Improved Optical Detection of Foodborne Pathogens |
| Author(s) |
Lixby Diaz-Amaya, Min Zhao, Li-Kai Lin, Carlos Ostos, Jan Allebach, George Chiu, Amanda Deering, Lia A. Stanciu |
| On-Site Speaker (Planned) |
Lixby Diaz-Amaya |
| Abstract Scope |
The increasing incidence of infectious outbreaks imposes a global burden for food safety, creating a market demand for on-site, disposable, easy-to-use and cost-efficient devices. This work aims to provide a cost-efficient, reliable and repeatable approach, for the detection of foodborne pathogens in real samples. We propose for the first time an optimized ink-jet printing platform, using a controlled nano-patterning of novel carboxyl-functionalized aptameric ink on nitrocellulose substrate for the highly efficient detection of E. coli O157:H7 (25 CFU ml-1 in pure culture and 233 CFU ml-1 in ground beef). This technology demonstrates the ability to control the variation within +/-1SD for at least 75% for data collected even at very low concentrations. To the best of our knowledge, this work reports the lowest LOD of the state-of-the art for paper-based optical detection of E. coli O157:H7, with enough evidence (p > 0.05) to prove its high specificity. |
| Proceedings Inclusion? |
Planned: Publication Outside of TMS |