| About this Abstract |
| Meeting |
MS&T23: Materials Science & Technology
|
| Symposium
|
Controlled Synthesis, Processing, and Applications of Structural and Functional Nanomaterials
|
| Presentation Title |
Nanostructured Surfaces for Quantitative Live Cell Microscopy Applications |
| Author(s) |
Michael Robitaille, Joseph Christodoulides, Jeff Byers, Marc Raphael |
| On-Site Speaker (Planned) |
Marc Raphael |
| Abstract Scope |
The integration of nanostructured surfaces with live cell microscopy has numerous important applications including cell secretion detection, directing cell morphology & migration and guiding stem cell differentiation, to name a few. However, live cell substrate surfaces are typically poorly characterized with regards to topography, mechanics and surface chemistries, leading to confounding results and a reputation for only giving qualitative biological insights. We present applications in which electron beam lithography is utilized to create gold nanostructure enhanced surfaces for quantitative in vitro microscopy applications. Surface plasmon resonance is subsequently employed to characterize the binding activity of the biofunctionalized gold surfaces. Example applications to be discussed include real time detection of antibody secretions from single hybridoma cells, label-free exosome detection with single exosome resolution, tuning of migratory phenotypes in breast cancer cells and directed fibroblast alignment and migration. |