| About this Abstract |
| Meeting |
MS&T24: Materials Science & Technology
|
| Symposium
|
Next Generation Biomaterials
|
| Presentation Title |
Chemical Modification of Polymers Using Gamma-Ray Irradiation for the Substrate of Stem Cell Culture |
| Author(s) |
Hideki Mori, Masayuki Hara |
| On-Site Speaker (Planned) |
Masayuki Hara |
| Abstract Scope |
We prepared covalently cross-linked poly (vinyl alcohol) (PVA) hydrogel using irradiation with gamma-ray. The gel is useful for adherent culture of neural stem/progenitor cells (NSPCs) for proliferation keeping their undifferentiated state. The cluster of NSPCs proliferated on the PVA gel. We also study the extracellular matrices (ECMs) involved in cell adhesion. We also mention another example of chemical modification polymers. The woven nylon mesh（NM） was modified with polyacrylic acid (PAA) by a graft-polymerization technique using the gamma-ray. Carboxyl residues of the PAA were chemically conjugated with Matrigel which is a mixture of ECM proteins, using the ECC/NHS reaction. This substrate is also useful for the adherent culture of immature NSPCs.
http://dx.doi.org/10.1016/j.jbiosc.2015.09.010 https://doi.org/10.1016/j.jbiosc.2020.12.003 |