| About this Abstract |
| Meeting |
2024 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
H-14: siRNA and Cerium Oxide Delivery With Ultrasound-sensitive Nanobubbles for Bone Disease Treatment |
| Author(s) |
Pedram Sotoudehbagha, Elayaraja Kolanthai, Fei Wei, Craig Neal, Udit Kumar, Melanie Coathup, Sudipta Seal, Mehdi Razavi |
| On-Site Speaker (Planned) |
Pedram Sotoudehbagha |
| Abstract Scope |
Ultrasound-sensitive nanobubbles (NBs) loaded with Cathepsin K short interfering RNA (CTSK siRNA) and cerium oxide nanoparticles (CNPs) were synthesized to downregulate the expression of CTSK in osteoclasts and improve the bone formation of human bone marrow-derived mesenchymal stem cells (hMSCs). Results showed that co-delivery of 2.5 nM CTSK siRNA and 1 µg/mL CNPs with NBs to hMSCs improved the alkaline phosphatase activity (ALP) and mineralization. The ALP activity and the optical density of alizarin red S (ARS) staining increased to 12.28 ± 4.68% vs 3.31 ± 0.61% for control (p < 0.05), and to 1.12 ± 0.03 vs 0.41 ± 0.02 for control (p < 0.001), respectively. Also, it reduced the number of TRAP-positive cells (15.4 ± 3.49/mm2 vs 26.73 ± 3.80/mm2 for the control, p < 0.01) in human osteoclast precursors which indicates lower osteoclastogenesis. Thus, this system can be a suitable carrier for bone treatment. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Nanotechnology, Other |