| About this Abstract |
| Meeting |
MS&T23: Materials Science & Technology
|
| Symposium
|
2023 Undergraduate Student Poster Contest
|
| Presentation Title |
Engineered Ex Vivo Co-culture Lung Model as a Platform for Testing Biomaterial-induced Cancer Cell-specific Cytotoxicity |
| Author(s) |
Miriam Stevens |
| On-Site Speaker (Planned) |
Miriam Stevens |
| Abstract Scope |
Current models to test the efficacy of anti-cancer drugs and adjuvants is limited as it relies on animal models that are associated with high costs and are time-consuming. Therefore, the objective of this summer research project was to (i) develop an ex vivo murine lung model using precision-cut lung slices (PCLS) in co-culture with human lung adenocarcinoma cells (A549) and (ii) test the cancer cell-specific cytotoxicity of drugs and materials-based agents. Our results show that murine cells within PCLS remain viable over at least five days in culture and support the adhesion and proliferation of A549 lung cancer cells. In addition, our preliminary data indicate that the treatment with anti-cancer agents maintains murine cell viability while inducing DNA damage in the human A549 cancer cells. Our future studies will test an array of anti-cancer agents and materials and establish the spatio-temporal relationship between treatment and cancer cell toxicity. |