| About this Abstract |
| Meeting |
2025 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
Identification of SARS-CoV-2 Virus Variants Through a Label-Free SERS Technique |
| Author(s) |
Han Lee, Jiunn-Der Liao, Wei-Chia Hsu |
| On-Site Speaker (Planned) |
Han Lee |
| Abstract Scope |
Since the spike protein structure of the SARS-CoV-2 virus affects its infectivity, there has been an increased need to differentiate SARS-CoV-2 viruses in clinical specimens. This study used a label-free surface-enhanced Raman scattering (SERS) method with porous and fibrous gold nanoparticles (Au NPs/pZrO2 and Au NPs/fZrO2) to detect Delta and Omicron variants. It analyzed six interfering substances and pre-treatment parameters to minimize spectral interference. Aliphatic structural vibrations (1100 cm-1) distinguished Delta and Omicron, while tyrosine vibrations (1400 cm-1) were specific to live viruses. Both substrates identified the inactivated Delta variant through specific spectral peaks despite interference. Clinical samples showed distinct peaks, including phenylalanine (1008 cm-1), L-arginine (1284 cm-1), and tyrosine (1592 cm-1). Proper pre-treatment with SERS methods reduced spectral interference, enhancing SARS-CoV-2 detection. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Nanotechnology, Other |