About this Abstract |
| Meeting |
2023 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
Influence of Fluid Flow on Inducing Bone Metastasis through use of a Novel Bioreactor with In Vitro Cancer Models |
| Author(s) |
Kalpana S. Katti, Haneesh Jssuja, Sharad Jaswandkar, Dinesh R Katti |
| On-Site Speaker (Planned) |
Kalpana S. Katti |
| Abstract Scope |
The WHO reports 375,304 deaths in 2020 due to prostate-cancer. Majority of these deaths occur due to bone metastasis. A novel nanoclay-based tissue-engineered polymeric scaffold sequentially seeded with hMSCs and cancer cells recapitulates prostate-cancer bone metastasis. Fluid-flow provides unique biochemical cues for metastasis. Hence, we designed a bioreactor that enables interstitial fluid-flow for prostate cancer bone metastasis. CFD results indicate that 0.05 ml/min flow rate recapitulates physiological condition. Also, the interstitial fluid-flow does not alter the CXCR4 level, an important regulator of metastasis and invasiveness, but bone proximity upregulates CXCR4 levels enabling increased MMP-9 levels. Further, v3 integrins and MMP-9 levels are upregulated by fluid-flow causing increased migration under fluid-flow. Our studies suggest that v3 integrins act as mechanosensory agents that transduce mechanical signals via v3-MMP 9 signaling to promote flow-induced motility of prostate cancer cells. Overall, these studies describe the critical role of interstitial fluid-flow in prostate cancer metastasis. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Characterization, Thin Films and Interfaces |