| About this Abstract |
| Meeting |
2024 TMS Annual Meeting & Exhibition
|
| Symposium
|
Advances in Biomaterials for 3D Printing of Scaffolds and Tissues
|
| Presentation Title |
Design and Optimization of a 3D-printed Bioreactor for Long-term Ex-vivo Bone Tissue Culture |
| Author(s) |
Anamika Prasad, Paula Gustin |
| On-Site Speaker (Planned) |
Anamika Prasad |
| Abstract Scope |
Studies of the effects of external stimuli on bone tissue, disease transmission mechanisms, and potential medication discoveries benefit from the long-term tissue viability ex-vivo. By simulating the in-vivo environment, bioreactors are essential for studying bone cellular activity throughout biological processes. We present the development of an optimized, automated 3D-printed bioreactor designed to sustain the ex-vivo viability of swine cancellous bone cores for an extended period. The optimized bioreactor system addresses several limitations of existing systems and provides a versatile platform for bone cancer research, orthopedic device testing, and other related applications. The bioreactor was tested both with culturing of fresh swine samples as well as with cell-laden 3D printed porous scaffold, with detailed quantification of cell viability and mineralization outcomes at several time points of storage. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Characterization, Mechanical Properties |