| About this Abstract |
| Meeting |
2022 TMS Annual Meeting & Exhibition
|
| Symposium
|
BioNano Interfaces and Engineering Applications
|
| Presentation Title |
K-14: AuNps Tailored Microneedle Based Point of Care Device Fabrication for Hormonal Sensing |
| Author(s) |
Shubhangi Shukla |
| On-Site Speaker (Planned) |
Shubhangi Shukla |
| Abstract Scope |
Leptin hormone levels are a vital biomarker for the glucose and fat metabolism in our body. The estimation of its concentration in blood is helpful for the prior diagnosis of chronic diseases. However, the process requires a selective and sensitive system for onboard detection in body fluids. Here, we intend to devise a noninvasive, eco-friendly, and imprint-free immunosensor based on ultrananocrystalline diamonds (UNCDs) microneedle array. The UNCDs surface is modified with an amphiphilic silane [(3-aminopropyltrimethoxysilane (3-APTMS)] functionalized gold nanoparticles (AuNPs) binded with monoclonal Anti-leptin receptor moieties for leptin transducers. The AuNPs were synthesized by chemical reduction method using 3-APTMS as a capping agent in the presence of formaldehyde as a reductant. The surface plasmons were coherently coupled with antibody molecules through glutaraldehyde and the silane effect on the electrode surface. Under NTP, the proposed immunosensor exhibited a wide linear range of 0.121–2500 pg/mL with a low detection limit of 0.025 pg/mL. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Nanotechnology, Thin Films and Interfaces |