| About this Abstract |
| Meeting |
2024 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
H-10: Cooperative Surface Self-assembly Kinetics of Metal Binding Peptides by Spectral Rate Distribution |
| Author(s) |
Taylor Bader, Kyle Boone, Christopher Johnson, Cindy L. Berrie, Candan Tamerler |
| On-Site Speaker (Planned) |
Taylor Bader |
| Abstract Scope |
Metal binding peptides (MBPs) that can recognize and bind to specific target metal surfaces with high affinity enable designing biohybrid interfaces with additional functionality. In the presence of their target surfaces, these peptides bind and self-assemble onto the surface through noncovalent interactions that are dynamic. Understanding the intricacies of this behavior of MBPs allows the design of biohybrid interfaces with predictable and tunable functional properties. We developed an adaptive model that fits experimental data using a cooperative binding approach. Peptide binding data was collected at different concentrations of MBPs using QCM-D and AFM to better understand the peptide’s assembly properties. Our model produces a spectrum of apparent rate constants that may provide guidance on the critical binding stages. Through this model, we improved the resolution of the kinetic constants of AuBP1, with an equilibrium constant to the order of 107, indicating the peptide’s potential in the development of picomolar technologies. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Thin Films and Interfaces, Modeling and Simulation, Biomaterials |