| About this Abstract |
| Meeting |
2022 TMS Annual Meeting & Exhibition
|
| Symposium
|
Functional Nanomaterials: Functional Low-Dimensional (0D, 1D, 2D) Materials 2022
|
| Presentation Title |
H-10: The Interaction Dynamics and Binding Strength of Proteins on Gold Nanoparticles |
| Author(s) |
Tushar Upreti |
| On-Site Speaker (Planned) |
Tushar Upreti |
| Abstract Scope |
Gold Nanoparticles (AuNP) have been touted for their high diagnostic and treatment potential. However, to predict the fate of NPs in biological milieu, we need to understand the interaction of AuNPs with vascular and extravascular proteins upon intravenous (IV) or intradermal (ID) administration, respectively. To investigate this protein-AuNP interaction, we first synthesized AuNP of two sizes (24nm and 38nm) using the citrate reduction technique. AuNPs were then exposed to either albumin or collagen individually, or sequentially to simulate IV and ID administration. UV-Vis and fluorescence spectroscopy combined with mathematical modeling was used to evaluate the protein-NP interaction. The results from this study show that the nature of protein-NP interaction is dynamic, and collagen has a higher binding affinity than albumin. Our study presents convincing data to suggest that the precoating of NPs with strong affinity proteins, such as collagen, confers predictive in vivo behaviour and greater NP stability. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Nanotechnology, Surface Modification and Coatings, Biomaterials |