| About this Abstract |
| Meeting |
MS&T22: Materials Science & Technology
|
| Symposium
|
Society for Biomaterials: Biological Response to Materials and Material’s Response to Biological Environments
|
| Presentation Title |
Effects of Phospholipid Saturation and Cholesterol Concentration on Liposome Interaction with Fungal and Mammalian Cells |
| Author(s) |
Veronica Lamastro, Kayla Campbell, Peter Gonzalez, Anita Shukla |
| On-Site Speaker (Planned) |
Veronica Lamastro |
| Abstract Scope |
Liposomes are lipid-based nanoparticles used to treat fungal infections with the potential to decrease cytotoxicity of antifungal drugs. Optimal liposomal formulations would preferentially target fungal cells over mammalian cells. Here, we investigated the effect of lipid saturation and cholesterol concentrations (15 – 40 w/w%) in liposomes on liposome-fungal and liposome-mammalian cell interaction. Generally, a high level of Candida albicans-liposome interaction was observed (>80%). Liposome interaction was observed with 10 – 50% of endothelial and 10 – 40% of fibroblast cells for monounsaturated formulations at different cholesterol concentrations; liposomes fabricated with saturated and polyunsaturated lipids showed approximately 100% interaction with mammalian cells. Additionally, formulations using unsaturated lipids mimicking the clinically used antifungal liposome AmBisome, typically fabricated with saturated lipids, had decreased levels of interaction with mammalian cells while maintaining high levels of C. albicans interaction. Overall, this work demonstrates the importance of lipid saturation in liposome compositions when designing antifungal liposomes. |