| About this Abstract |
| Meeting |
2023 Annual International Solid Freeform Fabrication Symposium (SFF Symp 2023)
|
| Symposium
|
2023 Annual International Solid Freeform Fabrication Symposium (SFF Symp 2023)
|
| Presentation Title |
Fabrication of Thick Vascularized Tissues using Sacrificial Ink-assisted Embedded 3D Bioprinting |
| Author(s) |
Bing Ren, Yong Huang |
| On-Site Speaker (Planned) |
Bing Ren |
| Abstract Scope |
Engineering three-dimensional (3D) in vitro vascularized tissue models remains a great challenge. Herein, a strategy integrating the sacrificial ink-assisted embedded bioprinting with cellular self-assembly is proposed to create thick vascularized tissues with macro- and micro-vessels. Perfusable channel structures are fabricated by embeddedly printing a sacrificial ink in an endothelial cell-laden and gelatin microgel-based yield-stress composite matrix. The sacrificial ink is removed to form channels as engineered macro-vessels after printing is completed and the matrix is crosslinked; micro-vessels are formed by self-assembly of encapsulated endothelial cells in the matrix. This embedded bioprinting approach has been implemented to print a composite matrix-based human alveolar model with vascularized air and fluid channels that are seeded with alveolar epithelial cells and endothelial cells, respectively, as well as composite matrix-based thick structured meat with macro-vessels seeded with endothelial cells and micro-vessels assembled by endothelial cells. |
| Proceedings Inclusion? |
Definite: Post-meeting proceedings |