| Abstract Scope |
Bone, a mineralized biological tissue, exhibits exceptional mechanical properties due to its hierarchical structures. This study employed Atomic Force Microscopy (AFM), Raman Spectroscopy, and Energy Dispersive X-ray Spectroscopy (EDS) to map the elastic modulus, phosphate/amide I ratio, and calcium and phosphorus elements, respectively. The results revealed a strong correlation between the elastic modulus and the phosphate/amide I ratio, as well as calcium and phosphorus concentrations within single trabeculae. Finite element models based on AFM images were then developed to explore the effects of various architectures on the mechanical behavior of trabecular bone. This combined experimental and computational approach enhances our understanding of bone mechanics at micro- and nano-scales, aiding in the prediction of bone fractures. These findings offer valuable insights for bioinspired material design, including engineered structures that mimic trabecular bone's architecture, with potential implications for biomedical applications, improving the understanding of bone-related diseases like osteoporosis, and optimizing treatments. |