| About this Abstract |
| Meeting |
2024 Annual International Solid Freeform Fabrication Symposium (SFF Symp 2024)
|
| Symposium
|
2024 Annual International Solid Freeform Fabrication Symposium (SFF Symp 2024)
|
| Presentation Title |
Greyscale DLP-Engineered Spatiotemporal Hydrogel Scaffolds |
| Author(s) |
Domenic Cordova, Maryam Tilton |
| On-Site Speaker (Planned) |
Domenic Cordova |
| Abstract Scope |
Digital Light Processing (DLP) presents a superior alternative to microextrusion in 3D bioprinting, offering high-resolution printing and structural fidelity. In particular, visible light-induced DLP (VL-DLP) enhances cell viability and functionality compared to UV photocrosslinking. This study introduces a novel approach by integrating greyscale VL-DLP with an engineered composite hydrogel formulation to develop spatiotemporal scaffolds aimed to guide cell migration, proliferation, and differentiation fate. We controlled the biophysical properties at each layer by adjusting light intensity to a pixel resolution of 35 µm. We used a photocrosslinkable ink composed of gelatin methacryloyl (GelMA) and polyethylene glycol diacrylate (PEGDA) at a 10% w/w concentration. Employing optical fiber-based interferometry nanoindentation, we have shown that precisely crafted scaffolds were achieved with gradient Young’s moduli varying from 15 to 160 kPa across each layer, demonstrating our technique’s potential for development of tailored therapeutics and more accurate in vitro 3D tissue modeling. |
| Proceedings Inclusion? |
Definite: Post-meeting proceedings |