| About this Abstract |
| Meeting |
2025 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
Fluid Flow Enabled Metastasis Testbed of Breast and Prostate Cancer Metastasis to Bone |
| Author(s) |
Kalpana S. Katti, Sharad Jaswandkar, Preetham Ravi, Shrinwanti Ghosh, Jiha Kim, Dinesh R Katti |
| On-Site Speaker (Planned) |
Kalpana S. Katti |
| Abstract Scope |
Tissues and cells experience shear stresses through interstitial fluid flow that contributes to the supply of nutrients, removal of waste, and movement of biomolecules through cells. We have developed a novel horizontal flow bioreactor that recapitulates the interstitial fluid flow conditions. The bioreactor includes a tissue engineered primary and metastasis sites models of breast and prostate cancer. Each channel in the bioreactor consists of primary site (EMT) chamber, secondary site (MET) chamber, cell/drug injection ports for inserting cells/drugs in the flow and sampling the fluid for extracting biomolecules or cells. We also generate a hypoxic environment in the bioreactor by treating prostate cancer cells with cobalt-chloride before injection. Overall, the EMT and MET characteristics as well as the flow of cells from primary site to secondary is captured using the bioreactor. Along with migration and transport of cancer cells this represents a robust in vitro testbed of cancer metastasis. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Composites, Polymers |