| About this Abstract |
| Meeting |
MS&T24: Materials Science & Technology
|
| Symposium
|
Nanotechnology for Energy, Environment, Electronics, Healthcare and Industry
|
| Presentation Title |
Multi-Scale Characterization of Riboflavin Supplements Using X-Ray Microscopy, SEM, and Automated Phase Analyses |
| Author(s) |
Andy Holwell, Ria Mitchell, Darragh Murnane |
| On-Site Speaker (Planned) |
Andy Holwell |
| Abstract Scope |
Riboflavin alleviates migraine symptoms through the reduction of neuroinflammation. The formulation includes anti-caking agents, fillers, and lubricants to maximize homogenization and bioavailability. Multi-scale characterization of supplements provides quality assurance and quantification of composition, distribution and morphology of components. Two riboflavin capsules presented very different efficacy; to understand this, novel 3D characterization techniques were used. Nanoscale X-ray microscopy was used to image the powders non-destructively in 3D, and apply advanced machine learning reconstruction to enhance visualization, improve phase contrast, and increase throughput. Automated phase classification quantified components in 3D. Additionally, correlative electron microscopy provided chemical analyses of the formulations. Each capsule’s powders exhibited large morphological and structural differences and we identified magnesium stearate binders and calcium phosphate fillers. Thus, non-destructive nanoimaging and AI-based image processing revealed previously unobservable phases. We identified and quantified components not listed as ingredients, demonstrating capability for quality assurance and regulation of supplements. |