| About this Abstract |
| Meeting |
2022 TMS Annual Meeting & Exhibition
|
| Symposium
|
BioNano Interfaces and Engineering Applications
|
| Presentation Title |
Peptide-mediated Remineralizable Collagen Fibrils as a Model for Dentin Repair |
| Author(s) |
Nilan Jayabahu Kamathewatta, Quang Ye, Paulette Spencer, Candan Tamerler |
| On-Site Speaker (Planned) |
Nilan Jayabahu Kamathewatta |
| Abstract Scope |
Collagen forms the major insoluble fibrous protein in most tissues. Type I collagen is the predominant organic component in dentin. Cariogenic bacteria demineralize and decompose dentin. Current treatment generally involves removal of caries-infected, but retention of caries-affected dentin. To date, the methods to repair damaged dentin have not consistently remineralized dentin. We used type I collagen as a model system to investigate peptide-mediated remineralization on self assembled collagen network. The morphologic and chemical changes in the collagen network in the presence and absence of hydroxyapatite-binding peptide were studied as a function of time following alkaline phosphatase exposure using AFM, micro-Raman spectroscopy, and SEM-EDX. With increased reaction time, the mineral particles form clusters, the phosphate band is better resolved, and carbonate content is decreased. These results indicate a higher degree of crystallinity and the results support physiological re-mineralizable collagen fibrils as a target for the repair of deficient dentin. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Characterization, Other |