| About this Abstract |
| Meeting |
2022 TMS Annual Meeting & Exhibition
|
| Symposium
|
Biological Materials Science
|
| Presentation Title |
Tuning the Cancer Microenvironment at Bone Metastasis Using the Cancer Testbed |
| Author(s) |
Kalpana S. Katti, Sharad Jaswandkar, Haneesh Jasuja, Hanmant Gaikwad, Farid M Solaymani, Jiha Kim, Anu Gaba, Dinesh R Katti |
| On-Site Speaker (Planned) |
Kalpana S. Katti |
| Abstract Scope |
Cancer that has metastasized to bone is essential incurable. The significantly altered environment at metastasis is responsible for the adhesion of cancer cells to bone and their proliferation at the bone-site. We have developed a nanoclay-based bone-scaffold for development of in vitro models of bone of breast and prostate cancer metastasis using commercial and patient-derived cancer cell lines. We use the testbed for screening drugs and discovery of metastasis-biomarkers. Adhesion forces between cancer cells and bone are measured using an aspiration-based AFM tip. This initial attachment is crucial for bone metastasis and progression of tumors. Mechanical cues are suggested by molecular simulations of integrins relevant to cancer cell attachment. Immunocytochemical imaging of cancer cells elucidates fundamental structural changes in the cells resulting from cell adhesion. Overall, we report the use of a cancer testbed to study progression of bone metastasis and for providing suitable mechanical and chemical cues against metastasis. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Modeling and Simulation, Mechanical Properties |