About this Abstract |
| Meeting |
MS&T24: Materials Science & Technology
|
| Symposium
|
3D Printing of Biomaterials and Devices
|
| Presentation Title |
Impact of Murine Cell Seeding on Vat Photopolymerized 3D Printed Scaffolds |
| Author(s) |
Abby Whittington, Sera Choi, Elizabeth Hunt, Edward Shangin, Zahra Bahranifard, Emma Nguyen, Caitlyn Collins |
| On-Site Speaker (Planned) |
Abby Whittington |
| Abstract Scope |
Vat photopolymerization (VP) is being investigated for use in bone tissue engineered scaffolds. VP uses light projected onto a photocurable resin to build layer-by-layer scaffolds with only a few biocompatible resins currently available. This study aims to determine the suitability of a soy-based resin blended with polyethylene glycol diacrylate (PEGDA) to support cell proliferation with biologically relevant mechanical properties. Three geometries were printed and their properties compared: Truncated Octahedron, Isotruss, and Voronoi Lattice. This first two are only achievable through 3D printing while the third mimics traditional scaffold geometries. Printed scaffolds were sterilized, seeded with MC3T3-E1 murine fibroblasts for up to a week and then fixed for imaging or mechanical testing. Unseeded scaffolds with matching geometry were used for controls. This work confirms soy-PEGDA resin can achieve high cell adherence and dynamic mechanical analysis confirms the storage moduli of the scaffolds are within range of soft fracture callus tissue. |