| About this Abstract |
| Meeting |
2025 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
Flavoprotein Oxidases Engineered with a Metal-Binding Peptide Tag as a Potential Diagnostic Enzyme |
| Author(s) |
Taylor Bader, Gabby LeDou, Candan Tamerler |
| On-Site Speaker (Planned) |
Taylor Bader |
| Abstract Scope |
Flavoprotein putrescine oxidase, a member of the oxidoreductase family, is regarded as a valuable biocatalyst. These enzymes facilitate the oxidation of various compounds while reducing oxygen to hydrogen peroxide. Polyamines, such as putrescine, naturally occur in living organisms and are involved in cell growth and differentiation. Elevated polyamine levels in body fluids are associated with several diseases, including cancer. Previously, we demonstrated that a gold-binding tagged putrescine oxidase can self-assemble on gold surfaces, enhancing association and maintaining catalytic activity. In this study, we explore the use of this fusion protein as a potential diagnostic enzyme in gold nanoparticle (AuNP) systems. We examined the system’s performance under different temperature and pH conditions and in the presence of other polyamines, both in-solution and immobilized on AuNPs. Our findings indicate that the peptide-tagged enzyme system broadens the effective temperature and pH range of the enzyme compared to the wild-type. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Nanotechnology, Characterization |